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HBV-DNA negativity, which is one of the major surrogate markers for HBeAg seroconversion, occurs at high rates in HBeAg-positive chronic hepatitis B (CHB) patients with prior lamivudine exposure, although the underlying mechanism has not yet been fully elucidated. The aim of this study is to clarify the mechanisms of HBeAg seroconversion. Sequences of the pre-S/S region of the HBV genome were analyzed by polymerase chain reaction in a total of 47 HBeAg-positive CHB patients who were treated with lamivudine and 9 untreated patients. Two pre-S/S deletion mutations associated with lamivudine resistance were detected in 14 of 47 patients (29.8%), including 12 that showed complete pre-S deletion, which was the most frequent mutant. Their HBsAg levels decreased to negative levels, and 23 (49.0%) subsequently became HBeAg-negative. Loss of pre-S was associated with high frequencies of TAC mutations at positions 49 and 53 (81.8%, 81.8%), which are major resistance-associated mutations at this position. It is concluded that HBsAg loss is the major cause of HBeAg seroconversion. The pre-S/S deletion mutations detected in this study have the potential for improving upon current antiviral treatment, and their removal represents an important therapeutic strategy for achieving complete HBeAg seroconversion. head on https://volektravel.com/tv-show-icon-pack-1-crack/
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