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\nIn vitro mutagenicity and cytotoxicity of deoxynucleoside 3-methyl-5-beta-d-riboside (3-Me-5′-rD), deoxycytidine 3-methyl-5-beta-d-riboside (3-Me-5′-dC), 2′-deoxyuridine 3-methyl-5-beta-d-riboside (3-Me-5′-U), 2′-deoxyadenosine 3-methyl-5-beta-d-riboside (3-Me-5′-Ade), 2-aminoadenine, and 3-aminoadenine were measured in the HPRT- and L5178Y TK-assays. All modifications result in a considerably enhanced mutagenicity in the HPRT-test. A significant, positive correlation was observed between mutagenicity in both assays, but cytotoxicity in the L5178Y TK-assay was not detected. The mutagenicity of 3-Me-5′-rD, 3-Me-5′-Ade, and 2-aminoadenine were increased at concentrations up to 10(-3) M. However, cytotoxicity was detected only at concentrations of 10(-4) M. 3-Me-5′-U and 3-Me-5′-dC were highly toxic at all concentrations investigated. UV-induced mutations at the hypoxanthine-guanine transition points were measured in the HPRT- and L5178Y TK-assays using Hprt- mutants of R1P2 strains. UV-induced mutation frequencies were only detectable in the HPRT-test. At all concentrations tested, UV-induced mutation frequency in the HPRT-test correlated well with mutagenicity in the HPRT-test. However, UV-induced mutation frequencies in the HPRT-test were considerably lower than mutagenicity in the L5178Y TK-test, indicating that mutagenicity in the L5178Y TK-test is due to the absence of repair processes.How to<\/p>\n","protected":false},"excerpt":{"rendered":"
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