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\nChlorpyrifos is a common agricultural insecticide which is reported to cause acute mortality in farmed fish and other aquatic animals. However, acute toxicity of chlorpyrifos to fish in the wild and under field conditions is largely unknown. We report here the results of an examination of acute oral toxicity of chlorpyrifos to tilapia (Oreochromis niloticus) and its 24h-LC50 was determined to be 0.61mg\/L. In an in vivo depuration study, 100% mortality in tilapia was observed within 4h after chlorpyrifos exposure at an LD50 of 0.30mg\/kg. To investigate whether metabolism is involved in acute toxicity of chlorpyrifos, we examined the effect of chlorpyrifos metabolism inhibitor, piperonyl butoxide, on tilapia. However, piperonyl butoxide did not attenuate the toxicity of chlorpyrifos, indicating that metabolism is not a major route for chlorpyrifos metabolism in tilapia. Results from exposure of fish to chlorpyrifos under field conditions indicated that 100% of the fish died within 12h after exposure to the field concentrations of chlorpyrifos. The oral toxicity of chlorpyrifos to tilapia, as determined under simulated field conditions, was more than 50-fold higher than observed under laboratory conditions. Simultaneous examination of chlorpyrifos metabolites indicated that chlorpyrifos metabolites are not elevated under field conditions, suggesting the possibility of field related stress.Preoperative multiplanar fluoroscopy-guided cyst-urethrogram for the evaluation of subfistula and extrinsic urethral obstruction in children.
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