{"id":7162,"date":"2022-06-10T04:27:34","date_gmt":"2022-06-10T07:27:34","guid":{"rendered":"http:\/\/www.vidriositalia.cl\/?p=7162"},"modified":"2022-06-10T04:27:34","modified_gmt":"2022-06-10T07:27:34","slug":"astroworld-2000-5-1-portable","status":"publish","type":"post","link":"https:\/\/www.vidriositalia.cl\/?p=7162","title":{"rendered":"Astroworld 2000 5.1 PORTABLE"},"content":{"rendered":"

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Astroworld 2000 5.1<\/p>\n

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boot to single user (root) mode
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* and *gfp^+^\/tm^2ls^* cohorts. Time-lapse microscopy analysis of the time of degradation of the nuclei marker DAPI. 20 or 28\u2009h after induction of the different expression vectors, z-stacks were taken every 20\u2009min and subjected to image processing. The cells were stained with DAPI to label nuclei and with Alexa Fluor 594 Phalloidin to label actin stress fibers. (**f**) Histogram of the nucleus degradation time in both cohorts. Each dot represents one cell. For each cell, the time point in which the nucleus is completely degraded is taken as the time of cell death. The solid line in the histogram represents the result of a linear fit to the distribution of cell death times.](ncomms14991-f7){#f7}
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