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Category:Data mining and machine learning softwareIsotopes in the measurement of the binding of monoclonal antibodies to cell-associated antigen.
The binding of monoclonal antibodies to a myeloid leukemia cell line (K562) has been measured using a 96-well microtiter plate format. The bound and the free fractions of antibody, i.e., the ratio of the number of unbound antibodies to the total antibodies bound to the target cells, was found to be the same when antibodies were added to the culture in the presence of 0.1% BSA or when BSA was included in the binding medium. The correlation between the measurements of the bound and free fractions of antibody was, however, significantly higher for BSA in the binding medium. In the absence of BSA, binding of antibody to the myeloid cells was found to be significantly higher at 4 degrees C than at 25 degrees C. However, the binding of the antibody to the cells was unchanged when the cells were incubated at 4 degrees C in the presence of BSA. It is concluded that BSA, in the concentration used, protects the antigenic epitopes of the cells from the inactivation that is caused by the low temperature, and that the difference between the measurements of the free and the bound fractions of the antibody is caused by a difference in the protection of the cells against the inactivation at 4 degrees C and 25 degrees C. This difference is therefore not related to differences in antibody binding to the antigens of the cells. It is also concluded that the ability of the microtiter plate to measure the fraction of bound antibody is sufficient to replace in vivo measurements of the binding of antibodies to cell-associated antigens.The present invention relates to a method for forming a stent-graft implant and more specifically to a method for forming a stent-graft by using a binder for the stent-graft to be formed.
The vascular system of human or animal bodies includes numerous blood vessels, from larger vessels to capillaries. These blood vessels are sometimes damaged, either by disease, such as arteriosclerosis, or by the effects of an injury, such as that associated with the use of a balloon catheter during angioplasty. When such damage or injury occurs, intravascular procedures such as percutaneous
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