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Phospholipase C was purified from Bacillus cereus by purification steps of heat treatment, chromatography on DEAE-Sepharose CL-6B, Sephadex G-100, and hydroxyapatite. Purified phospholipase C was separated into 4 subunits with molecular weight of 29,000 and 26,000 (alpha), 30,000 and 25,000 (beta), 24,000 and 22,000 (gamma), and 22,000 and 19,000 (delta), respectively, by SDS-polyacrylamide gel electrophoresis. The optimum pH of the enzyme was pH 7.5, and the temperature optimum was 60 degrees C, with the optimum temperatures of 40 degrees C and 70 degrees C at pH 6.5 and 7.5, respectively. Phospholipase C was not inhibited by EDTA and protamine sulfate. This enzyme hydrolyzed 1-palmitoyl-2-[14C]oleoyl-sn-glycero-3-phosphocholine, 3-[14C]palmitoyl-2-oleoyl-sn-glycerol 3-phosphocholine, 2-[14C]oleoyl-sn-glycerol 3-phosphocholine, 1-palmitoyl-2-[14C]oleoyl-sn-glycerol 3-phosphocholine, l-[3H]inositol 4-phosphate, l-[14C]phosphatidylinositol 4,5-bisphosphate, and 1-palmitoyl-2-[14C]oleoyl-sn-glycerol ditaurate but not l-[14C]phosphatidylethanolamine and l-[14C]phosphatidylserine.1955
September 21, 1955
Pastor Clarence B. Gates presents three important messages: 1. The Gospel of Christ; 2. The Controversy over the Decision to Separate Church and State; 3
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