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ProShow Producer Torrent
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The aim of this study was to compare four commercial systems for the detection of genomic DNA in total DNA, total RNA and in cDNA produced by two commercial reverse transcriptases. Moreover, we compared the efficiency of genomic DNA detection in cDNA when subjected to different extraction methods. Total DNA and total RNA samples were extracted from plasmid DNA containing a reference beta-galactosidase gene and cDNA was generated by a commercially available reverse transcription kit (Clontech). All four commercial systems were used for the detection of the genomic DNA. Genomic DNA in the DNA/RNA samples and in cDNA/cDNA hybrid samples was detected with all four commercial systems. No differences were found in detection sensitivity in any of the four systems. The performance of the systems differed according to the type of extract used (DNA/RNA or cDNA/cDNA hybrid). The optimal method for genomic DNA detection depended on whether the DNA/RNA or cDNA/cDNA hybrid was subjected to DNA extraction. The results of this study indicate that the commercial systems used are comparable with respect to detection
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