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* _Photoshop CS6: The Missing Manual,_ by Austin Shafrazi (O’Reilly)
* _Photoshop CS6 All-In-One For Dummies,_ by Matt Buzzell (Wiley)
* _Photoshop CS6: From Novice to Professional,_ by Mark Flomenbaum (Allworth Press)
* _Photoshop CS6: The Insider’s Guide to Mastering Digital Photo Editing,_ by Susan Derges (Allworth Press)
* _Photoshop CS6 Tutorials: A Beginner’s Guide to Photo Editing (Fourth Edition),_ by Eric Rosenquist (Wiley)
* _Photoshop CS6 for the Absolute Beginner: All You Need to Know to Start Editing,_ by Trevor Corbet (McGraw-Hill)
* _Adobe Photoshop CS6: Beginning Guide to Digital Image Editing,_ by Art Babbitt (Allworth Press)
* _Photoshop CS6: A Photo Editor’s Guide,_ by Bill Cairns (Wiley)
* _Photoshop CS6 Tutorial: Mastering Layers with the New Panel on Skill Set Six,_ by Steven McRoberts (allworth Press)
* _Photoshop CS6 Quick & Dirty Tips: A Shortcut Reference for Digital Photographers (Second Edition),_ by Steve Stavropoulos (Wiley)
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Elements is optimized for use with digital camera-mounted SLRs and DSLRs. The program imports many RAW formats including Adobe’s DNG and Lightroom’s ACR camera raw format (acquired directly from the camera). The application can be extended to incorporate custom actions which can be used to perform batch edits. It can also be connected directly to Lightroom through Adobe Camera Raw.
Adobe Photoshop Elements 2019: What’s new and how can you download?
Version 8.0 adds support for connecting Elements to Adobe Lightroom or the Adobe Creative Cloud for batch edits to be moved to Lightroom. Version 8.0 also adds the ability to work with RAW images without conversion to JPEG or TIFF. Adobe Photoshop Elements 2019 offers a new and improved interface with more editing features and the ability to split image modes and add watermarking for photos, as well as the ability to crop. The program also supports wireless networking.
Adobe Photoshop Elements 2019 offers a variety of new features such as: Add watermarking and auto crops to images with the new Crop Tool and Profile Tool. Add text and other effects with new text tools. Create and edit art brushes. The program’s cursor now works with touchscreen devices. Support for WDR (Wide Dynamic Range) and DR (Dynamic Range) formats for RAW images. In addition to supporting RAW image files, it also now supports RAW images in camera raw format (ACR).
Adobe Photoshop Elements 2019 is compatible with the following operating systems: Mac OS X and Windows. It is available for both download and use for a one-time fee as well as subscription. The program is compatible with iMac, Mac mini, Mac Pro, Retina MacBook Pro, MacBook, MacBook Air, Macbook Pro, and Macbook Air. To learn more about Photoshop elements 9.0, read its full review.
This version of Photoshop Elements is optimized for use with the following digital camera models: Nikon d610, Nikon d500, Nikon d5000, Nikon D5100, Nikon D5300, Nikon D5500, Nikon D5500, Nikon D90, Nikon D800, Nikon D810, Nikon D750, Nikon D3100, Nikon D3200, Nikon D5200, Nikon D5300, Nikon D5600, Nikon D610, Nikon D3300, Nikon D80, Nikon D40, Nikon D40x, Nikon D5100, Nikon D7000, Nikon D7
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Background
==========
Papillomavirus (PV) infection is an essential factor in the development of cervical cancer. PVs-induced cervical tumors are commonly driven by type 16 Human Papillomavirus (HPV16) that modifies host cell cycle through the expression of E6 and E7 oncoproteins.
Methods
=======
Exon 2 of the HPV16 E6 oncogene was cloned into an episomal vector pMEP4 to allow a doxycycline-regulated expression system. To monitor E6 transcriptional activity, the E6-dependent Luciferase (ELuc) gene was placed in the pMEP4 in frame with E6 sequences. An E6 sequence also contained the CMV promoter.
To determine the E6-mediated alteration of proliferation, and further check on the effect of E6 on cell cycle, a GFP-Rb-1 plasmid was used. Based on the “inducible or stable system” of HPV E6, the green fluorescent protein (GFP) was expressed by the CMV promoter in pEGFP-C1 under the control of the E6-dependent promoter. The pEGFP-C1 vector was then co-transfected with either pMEP4 (HPV16 episome) or pMEP4-E6 into HeLa cells. The co-transfection and the cell growth were both monitored with the epi-fluorescence, and the cell proliferation was assessed by determining the total cell number and the percentage of cells in different phases of cell cycle.
Results
=======
According to our results, we demonstrated that the E6 can promote cell proliferation by prolonging the G1 phase. We also detected that the E6 had the effect of the G1-S phase transition since E6 induced HeLa cells entering the G2-M phase at 24 hours, while expressing E7 caused slower cycling and more G0/G1 cells at 40 hours.
Conclusion
==========
We present here an “inducible” HPV16 E6 expression system in HeLa cells. The advantage of this system is that oncoproteins can be expressed and monitored simultaneously under the same cell cycle phase, while possible side effects can be ignored. Furthermore, the induction of E6 and/or E7 expression can be switched at any time by changing doxycycline concentration.
What’s New In?
SEOUL (Reuters) – Samsung Electronics Co Ltd forecast a rise in profit as prices for its components and displays improved, even as it warned of a tough road ahead for 2019.
The logo of Samsung Electronics is pictured at its headquarters in Seoul, South Korea, March 7, 2019. REUTERS/Kim Hong-Ji
Revenues at the world’s largest maker of smartphones and memory chips shrank in the fourth quarter, but the drop was smaller than market expectations, and operating profit at Samsung’s semiconductor unit showed signs of improvement.
Chief Financial Officer Yoon Ok-kyun told an earnings briefing on Wednesday that its main memory segment, which accounts for most of its earnings, saw a rise in profit, helped by better output.
Samsung’s main semiconductor business reported operating profit of 6.43 trillion won ($5.62 billion) in the October-December quarter, up from 5.81 trillion won a year earlier.
But chip prices in the fourth quarter were down on year-ago levels, and Samsung reiterated that it expected prices to stay volatile as trade tensions with Washington slowly simmer.
“As we see it, the semiconductor industry is facing such headwinds that it will be difficult to continue to improve its operational performance,” he said.
“We are not living in a time of a healthy semiconductor market.”
GAINS TO MAKE
Looking ahead to 2019, Samsung’s CFO and CEO of Samsung SDS, the company’s chip unit, Lee Hyeong-eun, said 2018 had been a year of “growth and profit improvement.”
This was driven by strong expansion in the smartphone market, which led Samsung’s memory unit to record $2.4 billion in operating profit, a 42% increase from a year earlier.
“I expect the year-on-year increase to continue,” Lee told the briefing, adding that the smartphone market would likely grow around 20% this year.
But he added that Samsung also expected a tough road ahead in 2019, forecasting a growth rate of “just around” 20% for memory sales, mainly due to “some slowing in China,” which accounts for half of its total.
Lee, in a graphic, showed a flattening smartphone market in China, where it is the number-one player, and growth
System Requirements:
Minimum:
OS: Windows 7 (SP1)
Processor: Intel Core 2 Quad Q9400 @ 2.66GHz or higher
Memory: 6 GB RAM
Graphics: DX10.0 compatible video card
Hard Drive: 50 GB free space
DirectX: Version 9.0
Network: Broadband Internet connection
Sound Card: DirectX 9.0 compatible Sound Card
Additional Notes: Requires DWM Windows Aero or Window 7 SP1 graphics mode.
Recommended:
OS: Windows 7
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