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[The detection of DNA damage using the single-cell gel electrophoresis of the comet assay]. The comet assay, in which the DNA of single cells is exposed to a single electric pulse and then spread on a glass plate and stained, is a simple, rapid and sensitive assay, which allows the detection of DNA damage in hundreds of individual cells. Moreover, it permits the detection of types of DNA damage and different topological forms of DNA damage in human cells in vivo, in comparison with the biochemical methods of study of DNA. It has also been demonstrated that the comet assay is suitable for the identification of cancer cells, since the DNA of cancer cells, due to the higher rate of DNA mutations in these cells, is more sensitive to damage than DNA from normal cells. This review article will present the details of the comet assay, current approaches to the isolation of peripheral blood lymphocytes, methods for detecting DNA damage and the different forms of DNA damage. IN THE UNITED STATES COURT OF APPEALS FOR THE FIFTH CIRCUIT United States Court of Appeals Fifth Circuit FILED September 11, 2009 No. 08-40684 Summary Calendar
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