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The amino-terminal region of the beta-subunit of Escherichia coli ribosomes, which contains an internal ribosomal entry site (IRES) that is responsible for translation initiation, has been located on peptides that allow its covalent attachment to Sepharose 4B. Upon incubation of these Sepharose conjugates with E. coli ribosomes in the presence of poly(U), the beta-subunit obtained was part of a large cross-linked polypeptide that was isolated by gel filtration and ion-exchange chromatography. This cross-linked product contained a cross-link between the C-terminus of the beta-subunit and a peptide at position 94 of the alpha-subunit. Mass spectrometry showed that the attachment site on the beta-subunit for this peptide was on the carboxyl side of Ala-64. No cross-links were observed between the beta-subunit and the ribosomal protein S2. Tryptic peptides corresponding to two ribosomal binding sites on the beta-subunit could be isolated using 4-(N-maleimidomethyl
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# No learning curveList of All Things Hotwheels
This is a list of all things Hot Wheels
The cars are listed in alphabetical order by marque
American Iron Team
American Iron Councilman Confetti Cruiser Special Edition (1998)
American Iron Councilman Confetti Cruiser Deluxe Edition (1999)
American Iron Councilman Confetti Cruiser Deluxe Vignette Edition (2000)
Austin Mini Countryman
Austin Mini Countryman (1998)
Big Bazooka
Big Bazooka (2002)
Big Bad Bug
Big Bad Bug (1999)
Bugatti T16
Bugatti T16 (1980)
Bugatti Veyron 16.4
Bugatti Veyron 16.4 (2002)
Cool Car
Cool Car (2000)
Crown Royal Pursuit
Crown Royal Pursuit (1996)
Cruiser
Cruiser (1966)
Cruiser Custom
Cruiser Custom (1986)
Cruiser Custom (1989)
Cruiser Custom (1992)
Cruiser Custom (1995)
Cruiser Magnum
Cruiser Magnum (1986)
Cruiser Magnum (1986)
Cruiser Magnum (1986)
Darrell Mini-Cruiser (2.0)
Darrell Mini-Cruiser (2.0)
Endurance
Endurance (1989)
Endurance (2.0)
Endurance (2.0)
Hula Hoop
Hula Hoop (2000)
Iron Witch II
Iron Witch II (2000)
Krause
Krause Copperhead (1990)
Lemon Shark I
Lemon Shark I (1998)
Lemon Shark II
Lemon Shark II (2000)
Lemon Shark II (2.0)
Lemon Shark II (2.0)
Lemon Shark II (2.0)
Lemon Shark II (2.0)
Lemon Shark II (
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DirectX: Version 11 or higher.
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Additional Notes:
Mac users require a Mac keyboard.
The font used is “Apple Chancery
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