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Sharing is caring. We hope you found the information provided useful and will share this list with a friend or loved one.Our long-term goal is to understand the role of the apicomplexan actin- myosin motor, Glideosa (G), for the gliding motility of the apicomplexan parasitophorous vacuole (PV) and the contractile dynamics of the apical complex (APC) that envelops these events. To address this objective, we took a genetic and biochemical approach. We created conditional mutants for glideosa and other components of the apicomplexan actomyosin motor and assayed them for motility and the ability of their PV to maintain a permissive apical microenvironment for intra-vacuole invasion by intraerythrocytic stages. We have identified two conserved apicomplexan actin-binding proteins that are essential for gliding motility and which, therefore, are likely members of the motor complex. One is a new isoform of the LIM-domain protein, Mbs, which is essential for motility and which stabilizes M2AP and F-actin and may play a role in flagellar motility. The other is an isoform of the conserved ADF/cofilin protein, Moco, which forms a complex with G and M2AP, and which likely helps to control the formation and/or function of the apical complex during gliding. Our findings show that glideosa is a dimer, and we have made an anti-G2A monoclonal antibody that is specific for glideosa, and that blocks glideosa in motility. This antibody was used to show that G is required for gliding and that it localizes to the gliding apparatus. Together, our discoveries point to G as a critical component of the gliding motor complex and suggest a new role for the APC during gliding. We plan to identify new components of the gliding motor complex that interact with G and to determine what their roles are during gliding motility and in maintenance of the apical microenvironment. We will test the idea that the apicomplexan ADP-ribosylation system controls gliding motility and the apical micro
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